PharmaTOX: Temporal and spatial variation in pharmaceutical concentrations in urban and agricultural stream: resulting effects on periphytic and sediment microbial communities

Coordinator: Stéphane Pesce (INRAE – UR RiverLy, EMA)

Partner PIs:

• Stéphane Pesce & Chloé Bonnineau (INRAE – EMA, UR RiverLy, Lyon)
• Aymeric Dabrin, Christelle Margoum, Matthieu Masson & Cécile Miège (INRAE – LAMA, UR RiverLy, Lyon)
• Emmanuel Naffrechoux (USMB – UMR EDYTEM, Le Bourget du lac)
• Emilie Lyautey (USMB – UMR CARRTEL, Le Bourget du lac)
• Ed Topp (Agriculture and Agri-Food Canada, London, Ontario, Canada)

Two study sites:

Ardières River, LTER Ardières-Morcille, Beaujolais vineyard area

Tillet River, tributary of Lake Bourget (Savoie)

Main objectives of PharmaTOX:

• to describe the spatial and seasonal gradient of pharmaceutical substances in two study sites, in comparison to more “historical” pollution (pesticides and metals for Ardières; PCBs for Tillet);
• to characterize the distribution of different pharmaceutical substances between the surface water and surface sediments;
• to evaluate the consequences on the benthic microbial communities exposed in these two compartments in terms of antimicrobial resistance occurrence and of functional tolerance acquisition (PICT).